CERK Rabbit Polyclonal Antibody

CAT#: AP05212PU-N

CERK rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western Blot: 1 - 10 µg/ml.
  Positive Control: High level expression in heart, brain, skeletal muscle, kidney and liver.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from human ceramide kinase protein
• Specificity: This antibody reacts to Ceramide Kinase.
• Formulation: Phosphate buffered saline with 0.08% sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: Lot specific
• Gene Name: Homo sapiens ceramide kinase (CERK)
• Database Link:
  Entrez Gene 64781 Human
• Background: Sphingolipids, in addition to being structural components of membranes, regulate cell-cell and cell-substrate interactions, proliferation, and differentiation. Members of this diverse group of lipids have emerged as a novel class of signaling molecules that also regulate phagocytosis. The mechanisms by which sphingolipids exert these effects remain incompletely defined. More than a decade ago, it was found that ceramide can be phosphorylated to ceramide 1-phosphate (C1P). Ceramide kinase (CERK) and its phosphorylated product ceramide 1-phosphate (C1P) are central players in inflammation and cancer. The product of CERK activity, ceramide 1-phosphate (C1P), has been reported to have mitogenic effects. C1P is a direct activator of cytosolic phospholipase A2 and is involved in arachidonic acid release. CERK is a mediator of Ca2+-dependent degranulation in mast cells. In both arachidonic acid release and mast cell degranulation, the intracellular elevation of Ca2+ is a central event that acts as a regulatory mechanism of CERK activity. C1P is found in brain synaptic vesicles, and plays a role in regulating the secretion of neurotransmitters. CERK activity exists in HL-60 cells where the C1P is derived from ceramide released from sphingomyelin. The expressed kinase has specific ceramide phosphorylating activity. CERKs exist in a variety of cellular organisms, including plants, nematodes, insects, and vertebrates.
• Synonyms: hCERK, Acylsphingosine kinase, Lipid kinase 4, KIAA1646, LK4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism