Sphk1 pSer225 Rabbit Polyclonal Antibody

CAT#: AP05255PU-N

Sphk1 pSer225 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot using anti-Mouse SphK1 (pSer225) on Mouse B-cell lysate. Antibody used at 1 ug/ml with phosphorylated blocking peptide (Lane A) and without (Lane B).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1-5 µg/ml.
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide incorporating the Serine 225 phosphorylation site of Mouse Sphingosine kinase 1
• Specificity: This antibody detects Sphingosine Kinase 1 (pSer225).
  Antibody shows some cross reactivity (~10% by ELISA) with other phosphoserine containing proteins.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.08% sodium azide.
• Concentration: 0.45 mg/ml
• Purification: Antigen Immunoaffinity Purification
• Database Link:
  Entrez Gene 20698 Mouse
• Background: Activation of Sphingosine kinase (Sphk) increases the bioactive lipid, sphingosine 1-phosphate (S1P) and has been observed in a variety of cancer cells. Phosphorylation of sphingosine by sphingosine kinase (Sphk) is the rate-limiting step in the cellular synthesis of S1P. A principle activation event is the phosphorylation of serine 225 of sphiongosine kinase 1. Inhibition of Sphk1 phosphorylation and hence, Sphk activity is an important target for the development of drugs for cancer, inflammation, cardioprotection as well as a many other indications.
• Synonyms: SPHK, SPK, SPK 1, SPK-1