SPARC Rabbit Polyclonal Antibody

CAT#: AP05261PU-N

SPARC rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot: Detection of endogenous SPARC in mouse heart lysate using (0.75 ug/ml) and anti-rabbit HRP (1:75k) and using Pierce's Super Signal West Femto.

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  Immunohistochemistry: Detection of endogenous SPARC in human hepatocellular carcinoma (HCC). 2 ug/ml, developed with Vector ImmPress anti-rabbit HRP and DAB substrate.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot: 0.5-1 µg/ml.
  Positive Control: Mouse heart lysate.
  Immunohistochemistry.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from the Human SPARC protein
• Specificity: This antibody detects Human SPARC.
  May cross react with SPARCL1.
• Formulation: Phosphate buffered saline with 0.08% Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Antigen Immunoaffinity Chromatography
• Storage: Upon arrival freeze at -20°C to -70°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens secreted protein acidic and cysteine rich (SPARC), transcript variant 1
• Database Link:
  Entrez Gene 20692 MouseEntrez Gene 6678 Human
• Background: SPARC is a key factor in cell-matrix interactions and possibly tumour aggressiveness. The SPARC gene, which encodes a multifunctional glycoprotein with roles in tissue development, remodelling and fibrosis. A regulator of cell-extracellular matrix (ECM) interactions, SPARC represents a major factor in the ECM remodelling occurring during tumour invasion. in silico analysis reveals 4 UTR-SNPs located in the 3 -UTR of the SPARC gene, corresponding to 1474 g a, 1551 g c, 1922 t g and 2072 c t changes, which are significantly associated with tumoral state of the tissue. Of all hits, the 2072 SPARC polymorphism had the best association with cancer.
  SPARC therefore is a gene involved in a number of diseases including rheumatoid arthritis, scleroderma, tumor development and metastasis. SPARC variants have been detected in tumour samples of patients with acute myeloblastic leukemia (AML).
• Synonyms: ON, Basement-membrane protein 40, BM-40

Reference Data

• Protein Families: Druggable Genome