Col3a1 Rabbit Polyclonal Antibody

CAT#: AP05390PU-N

Col3a1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC
• Recommended Dilution: Immunohistochemistry on frozen sections.
  ELISA: 1/1500 - 1/6000.
  Immunofluorescence.
• Reactivities: Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Collagen type III from rat tail tendon
• Specificity: This antibody is specific for rat Collagen 3 binding to both native and heat denatured collagen 3. This antibody is reactive with rat kidney, liver, skin and heart, but does not stain basement membranes.
• Formulation: Contains mannitol, dextran and salts
  State: Purified
  State: Lyophilised purified IgG
• Reconstitution Method: Reconstitute in 0.5 ml of distilled water.
• Concentration: 0.1 mg/ml
• Gene Name: Rattus norvegicus collagen type III alpha 1 chain (Col3a1)
• Database Link:
  Entrez Gene 84032 Rat
• Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues.
  Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
• Synonyms: COL3A1