FLIP (CFLAR) (C-term) Rabbit Polyclonal Antibody

CAT#: AP05788PU-N

FLIP (CFLAR) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K0) and NIH/3T3 cells probed with Rabbit anti FLIP gamma/delta

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  Immunocytochemical staining of HeLa cells with Rabbit anti FLIP gamma/ delta (A)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections: 2 µg/ml.
  Western blot: 1:1000; detects bands of approximately 35kDa and 25kDa in Jurkat cell lysates.
  Immunocytochemistry.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A peptide corresponding to aa191 - 209 at the C-terminus of human FLIP delta/FLIPs form.
• Specificity: This antibody recognises the apoptosis related protein designated FLIP (FLICE inhibitory protein).
• Formulation: Phosphate buffered saline containing 0.02% Sodium Azide (NaN3)
  State: Purified
  State: Liquid purified IgG
• Concentration: 1.0 mg/ml
• Gene Name: Homo sapiens CASP8 and FADD like apoptosis regulator (CFLAR), transcript variant 2
• Database Link:
  Entrez Gene 12633 MouseEntrez Gene 8837 Human
• Background: FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1.
  FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. AHP1591 recognizes human and murine delta and gamma variants but not the alpha or beta, variants.
  FLIP is widely expressed in human tissues and a diverse range of cell lines.
• Synonyms: c-FLIP, CLARP, MRIT, CASH, FLAME-1, CASP8AP1, Usurpin

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Apoptosis