AMPK alpha 1 (PRKAA1) Rabbit Polyclonal Antibody

CAT#: AP06008PU-N

AMPK alpha 1 (PRKAA1) rabbit polyclonal antibody, Aff - Purified


USD 370.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Applications ELISA, IF, IHC, WB
Recommended Dilution Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin sections: 1/50-1/200.
Immunofluorescence: 1/50-1/200.
Reactivities Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide, corresponding to amino acids 450-500 of Human AMPKα1.
Specificity This antibody detects endogenous levels of PRKAA1 / AMPK1 protein.
(region surrounding Thr479)
Formulation Phosphate buffered saline (PBS), pH 7.2
State: Aff - Purified
State: Liquid purified Ig fraction
Preservative: 0.05% Sodium azide
Concentration 1.0 mg/ml
Purification Affinity chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Predicted Protein Size ~ 63 kDa
Gene Name Homo sapiens protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant 1
Background AMPK is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.
Synonyms AMPK1, AMPK alpha-1 chain
Reference Data
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.