Cullin 1 (CUL1) Rabbit Polyclonal Antibody

CAT#: AP06077PU-N

Cullin 1 (CUL1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CUL-1 antibody (Cat.-No.: AP06077PU-N) at 1/500 dilution Lane 1:MCF-7 cell lysate Lane 2:NIH-3T3 cell lysate Lane 3:Rat kidney tissue lysate

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  Immunohistochemical analysis in paraffin-embedded human lung carcinoma tissue using Cullin-1 antibody (Cat.-No. AP06077PU-N).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Cullin-1 protein (region surrounding Tyr761).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE)
• Predicted Protein Size: ~90 kDa
• Gene Name: Homo sapiens cullin 1 (CUL1)
• Database Link:
  Entrez Gene 26965 MouseEntrez Gene 8454 Human
• Background: CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase.
• Synonyms: Cullin-1, CUL-1, CUL1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway