E2F6 Rabbit Polyclonal Antibody

CAT#: AP06092PU-N

E2F6 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of E2F-6 antibody (Cat.-No.: AP06092PU-N) at 1/500 dilution Lane 1:Hela cell lysate Lane 2:Mouse muscle tissue lysate Lane 3:Rat heart tissue lysate

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  Immunohistochemical analysis in paraffin-embedded human brain tissue using E2F6 antibody (Cat.-No. AP06092PU-N).

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution:

  Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.

• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of E2F6 protein (region surrounding Glu170).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE).
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~31 kDa
• Gene Name: Homo sapiens E2F transcription factor 6 (E2F6), transcript variant a
• Database Link:
  Entrez Gene 50496 MouseEntrez Gene 313978 RatEntrez Gene 1876 Human
• Background: The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.
• Synonyms: E2F-6, MGC111545

Reference Data

• Protein Families: Transcription Factors