Ezrin (EZR) Rabbit Polyclonal Antibody

CAT#: AP06112PU-N

Ezrin (EZR) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in extracts from NIH/3T3 cells using Ezrin/Villin-2 antibody.

• 

  Immunohistochemical analysis in paraffin-embedded human brain tissue using Ezrin/Villin-2 antibody ( ).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.

• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 500-550 of Human Ezrin.
• Specificity: This antibody detects endogenous levels of Ezrin / Villin-2 protein (region surrounding Ser539).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (>95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~69, 81 kDa
• Gene Name: ezrin
• Database Link:
  Entrez Gene 7430 Human
  UniProt ID P15311
• Background: Ezrin, Moesin and Radixin belong to a family of highly homologous Actinassociated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation.
• Synonyms: p81, Cytovillin, Villin-2, EZR, VIL2