GDNF Receptor alpha 1 (GFRA1) Rabbit Polyclonal Antibody

CAT#: AP06136PU-N

GDNF Receptor alpha 1 (GFRA1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of GFRa-1 antibody at 1/500 dilution Lane 1:HEK293T whole cell lysate Lane 2:Mouse liver tissue lysate Lane 3:PC12 whole cell lysate Lane 4:H9C2 whole cell lysate

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  Immunohistochemistry analysis of GFRA1 / GDNFR-alpha Antibody in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human GFRα-1.
• Specificity: This antibody detects endogenous levels of GFR alpha-1 protein.
  (region surrounding Lys90)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 51 kDa
• Gene Name: Homo sapiens GDNF family receptor alpha 1 (GFRA1), transcript variant 1
• Database Link:
  Entrez Gene 14585 MouseEntrez Gene 25454 RatEntrez Gene 2674 Human
• Background: Glial cell line-derived neurotrophic factor (GDNF) and the related neurotrophic factor neurturin (NTN) are potent survival factors for central and peripheral neurons. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGFβ superfamily of growth factors. Three receptors for these factors, GFRα-1 (also designated GDNFR-α, RETL1 or TrnR-1), GFRα-2 (also designated GDNFR-β, RETL2, NTNR-α or TrnR-2) and GFRα-3 have been identified. The receptors do not contain transmembrane domains and are attached to the cell membrane by glycosyl-phosphoinositol linkage. Both GFRα-1 and GFRα-2 have been shown to mediate the GDNF-dependent and NTN-dependent phosphorylation and activation of the tyrosine kinase Ret. GFRα-3 is expressed only during development.
• Synonyms: GFR-alpha-1, GDNF receptor alpha, RET ligand 1, GDNFRA, RETL1, TRNR1

Reference Data

• Protein Families: Druggable Genome