Cytokeratin 16 (KRT16) Rabbit Polyclonal Antibody

CAT#: AP06197PU-N

Cytokeratin 16 (KRT16) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in extracts from HepG2 cells using Cytokeratin 16 antibody.

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  Immunohistochemistry analysis in paraffin-embedded human breast carcinoma tissue using Cytokeratin 16 antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 421-470 of Human Cytokeratin 16.
• Specificity: This antibody detects endogenous levels of Cytokeratin 16 protein.
  (region surroundign Ser451)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Stabilizer: 50 % Glyerol
  Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~52 kDa
• Database Link:
  Entrez Gene 3868 Human
• Background: Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.
  Two cytokeratins, 6 and 16, are expressed when keratincytes are undergoing rapid turnover in various pathological states, wound healing, psoriasis and some carcinomas
• Synonyms: Cytokeratin-16, Keratin-16, Keratin 16, KRT16, KRT16A, CK16, K16