Cytokeratin 7 (KRT7) Rabbit Polyclonal Antibody

CAT#: AP06204PU-N

Cytokeratin 7 (KRT7) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry analysis of Cytokeratin 7 antibody (Cat.-No AP06204PU-N) in paraffin-embedded human breast carcinoma tissue.

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  Immunohistochemistry analysis of Cytokeratin 7 antibody (Cat.-No AP06204PU-N) in paraffin-embedded human breast carcinoma tissue at 1/100.

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  Western blot analysis of Cytokeratin 7 antibody Cat.-No AP06204PU-N in extracts from HepG2 cells.

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  Western blot (WB) analysis of Cytokeratin 7 antibody (Cat.-No AP06204PU-N) at 1/500 dilution. Lane 1: SGC7901 whole cell lysate(40ug). Lane 2: A431 whole cell lysate(10ug). Lane 3: PC12 whole cell lysate(40ug). Lane 4: SP2/0 whole cell lysate(40ug).

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  Western blot analysis of Cytokeratin 7 antibody (Cat.-No AP06204PU-N) at 1/500 dilution in HeLa (Lane 1), sp2/0 (Lane 2) and H9C2 (Lane 3) whole cell lysates.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 411-460 of Human Cytokeratin 7.
• Specificity: This antibody detects endogenous levels of Keratin 7 protein.
  (region surrounding Leu451)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~51 kDa
• Gene Name: keratin 7
• Database Link:
  Entrez Gene 3855 Human
  UniProt ID P08729
• Background: Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane.
• Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin