NF-kB p65 (RELA) Rabbit Polyclonal Antibody

CAT#: AP06247PU-N

NF-kB p65 (RELA) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of NFkB-p65 antibody at 1/500 dilution Lane 1:Hela whole cell lysate Lane 2:Mouse liver tissue lysate Lane 3:Rat kidney tissue lysate

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  Immunohistochemistry (IHC) analyzes of NF?B-p65 antibody in paraffin-embedded human breast carcinoma tissue

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 220-270 of Human NFkB-p65.
• Specificity: This antibody detects endogenous levels of NFkappaB-p65 protein.
  (region surrounding Val248)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 60, 75 kDa
• Gene Name: Homo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 1
• Database Link:
  Entrez Gene 19697 MouseEntrez Gene 309165 RatEntrez Gene 5970 Human
• Background: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFkappaB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp kappaB sequence in the immunoglobulin kappa light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFkappaB is activated and NFkappaB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
• Synonyms: NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway