Dynamitin (DCTN2) Rabbit Polyclonal Antibody

CAT#: AP06265PU-N

Dynamitin (DCTN2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p50 Dynamitin antibody in extracts from A549 cells.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 340-390 of Human Dynactin 2.
• Specificity: This antibody detects endogenous levels of p50 Dynamitin protein.
  (region surrounding Leu371)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Predicted Protein Size: ~ 44 kDa
• Gene Name: Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 1
• Database Link:
  Entrez Gene 69654 MouseEntrez Gene 299850 RatEntrez Gene 10540 Human
• Background: Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This ; is against the full length p50 dynamitin.
• Synonyms: DCTN2, DCTN50, Dynactin 2, p50 dynamitin, RBP50

Reference Data

• Protein Pathways: Huntington's disease