c Rel (REL) Rabbit Polyclonal Antibody

CAT#: AP06306PU-N

c Rel (REL) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of c-Rel antibody at 1/500 dilution Lane 1:Raw264.7 whole cell lysate Lane 2:Mouse heart tissue lysate Lane 3:Rat heart tissue lysate Lane 4:Rat brain tissue lysate

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  Immunohistochemistry (IHC) analyzes of Rel antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 470-520 of Human c-Rel.
• Specificity: This antibody detects endogenous levels of Rel protein.
  (region surrounding asn497)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 68 to 78 kDa
• Gene Name: Homo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog (REL), transcript variant 1
• Database Link:
  Entrez Gene 5966 Human
• Background: c-Rel is the cellular cognate of v-Rel, the avian reticuloendotheliosis virus strain T transforming gene. v-Rel encodes a phosphoprotein that is located in the cytoplasm of transformed spleen cells and in the nucleus of non-transformed fibroblasts, in contrast to the c-Rel protein, which is cytoplasmic. c-Rel has been shown to represent a constituent of the κB site binding transcription factor NFκB, which plays a crucial role in the expression of immunoglobulin κ light chain gene. In contrast to c-Rel, v-Rel is truncated in its C-terminal transactivation domain and does not appear to function as a transcriptional transactivator. It has thus been postulated that v-Rel may interfere with the normal transcription of NFκB regulated genes and thus cause transformation by a mechanism analogous to v-ErbA, which binds to the thyroid hormoneresponsive region in certain erythroid genes needed for differentiation, but cannot be activated by thyroid hormone.
• Synonyms: c-Rel

Reference Data

• Protein Families: Druggable Genome, Transcription Factors