SHP2 (PTPN11) Rabbit Polyclonal Antibody

CAT#: AP06315PU-N

SHP2 (PTPN11) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of SH-PTP2 antibody in extracts from hela cells.

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  Immunohistochemical analysis in paraffin-embedded human brain tissue using PTPN11 / SH-PTP2 antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 320-370 of Human SHP-2.
• Specificity: This antibody detects endogenous levels of PTPN11 / PTP2C / SH-PTP2 protein (region surrounding Ser350).
• Formulation: Phosphate buffered saline (PBS), approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen (purity > 95% by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 70 kDa
• Gene Name: protein tyrosine phosphatase, non-receptor type 11
• Database Link:
  Entrez Gene 19247 MouseEntrez Gene 25622 RatEntrez Gene 5781 Human
  UniProt ID Q06124
• Background: Several groups have independently identified a non-transmembrane PTP, designated SH-PTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. SH2 domains generally mediate the association of regulatory molecules with specific phosphotyrosine-containing sites on autophosphorylated receptors, thereby controlling the initial interaction of receptors with these substrates. A second and much more widely expressed PTP with SH2 domains, SH-PTP2 (also designated PTP1D and Syp), has been identified. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (CSW) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog.
• Synonyms: SHPTP2, PTP-1D, PTP-2C, SH-PTP2, SHP-2, Shp2, SH-PTP3

Reference Data

• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Adipocytokine signaling pathway, Chronic myeloid leukemia, Epithelial cell signaling in Helicobacter pylori infection, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Renal cell carcinoma