TGF beta 1 (TGFB1) Rabbit Polyclonal Antibody

CAT#: AP06350PU-N

TGF beta 1 (TGFB1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of TGFβ1 antibody at 1/500 dilution Lane 1:Hela cell lysate Lane 2:SP2/0 cell lysate Lane 3:H9C2 cell lysate Lane 4:MCF-7 cell lysate

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  Immunohistochemistry (IHC) analyzes of TGF-beta1 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 341-390 of Human TGFβ1.
• Specificity: This antibody detects endogenous levels of TGF beta1 protein.
  (region surrounding Tyr369)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~44.0 kDa
• Database Link:
  Entrez Gene 7040 Human
• Background: Transforming growth factor betas (TGFbetas) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFalpha. It is now realized that TGFbetas mediate many cell-cell interactions that occur during embryonic development. Three TGFbetas have been identified in mammals. TGFbeta1, TGFbeta2 and TGFbeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGFbeta requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGFbeta3 protein has approximately 80% identity to the mature region of both TGFbeta1 and TGFbeta2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity.
• Synonyms: TGFB, Transforming growth factor beta-1, TGF-beta-1