Glucocorticoid Receptor (NR3C1) Rabbit Polyclonal Antibody

CAT#: AP06390PU-N

Glucocorticoid Receptor (NR3C1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of GR antibody in extracts from Jurkat cells treated with EGF 200ng/ml 15'.

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  Immunohistochemistry (IHC) analyzes of GR antibody in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Porcine, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human GR.
• Specificity: This antibody detects endogenous levels of GR protein.
  (region surrounding Leu224)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 90,95 kDa
• Gene Name: Homo sapiens nuclear receptor subfamily 3 group C member 1 (NR3C1), transcript variant 1
• Database Link:
  Entrez Gene 14815 MouseEntrez Gene 24413 RatEntrez Gene 2908 Human
• Background: The glucocorticoid receptor (GR) is an ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GRα. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis and cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the nonligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active.
• Synonyms: NR3C1, GR, GRL

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Neuroactive ligand-receptor interaction