EDD (UBR5) Rabbit Polyclonal Antibody

CAT#: AP06447PU-N

EDD (UBR5) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of EDD antibody in extracts from A549 cells.

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  Immunohistochemistry (IHC) analyzes of EDD antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 1-50 of Human HYD.
• Specificity: This antibody detects endogenous levels of EDD protein.
  (region surrounding Thr14)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Predicted Protein Size: ~ 309 kDa
• Gene Name: Homo sapiens ubiquitin protein ligase E3 component n-recognin 5 (UBR5), transcript variant 1
• Database Link:
  Entrez Gene 70790 MouseEntrez Gene 51366 Human
• Background: EDD (for E3 identified by Differential Display) is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth (2). EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.
• Synonyms: EDD, EDD1, KIAA0896

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis