CD130 (IL6ST) Rabbit Polyclonal Antibody

CAT#: AP06506PU-N

CD130 (IL6ST) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of gp130 antibody in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of gp130 antibody in paraffin-embedded human breast tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 750-800 of Human gp130.
• Specificity: This antibody detects endogenous levels of CD130/gp130 protein.
  (region surrounding Ser778)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~160 kDa
• Database Link:
  Entrez Gene 3572 Human
• Background: IL-6 activates intracellular signaling through binding a receptor consisting of an 80 kDa ligand-binding protein (IL-6R) and a second protein of 130 kDa. IL-6 first binds to IL-6R which subsequently associates with a gp130 dimer. The active signaling complex consists of at minimum IL-6, IL-6R and a dimer of two gp130 proteins that are linked by a disulfide bond. A soluble form of IL-6R is generated by proteolytic cleavage of the membrane-bound precursor and can function as an agonistic molecule that can actively participate in cell-tocell signaling. The second subunit of the IL-6 complex, gp130, also functions as a component of several additional receptor complexes including leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and IL-11. LIF binds to the LIF receptor with low affinity and to a complex of the LIF receptor and gp130 with high affinity, while OSM appears to bind to gp130 with low affinity and to a complex of gp130 and the LIF receptor with high affinity.
• Synonyms: Interleukin-6 receptor subunit beta, IL-6R-beta, IL6 receptor beta