Collagen I (COL1A2) Rabbit Polyclonal Antibody

CAT#: AP06516PU-N

Collagen I (COL1A2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of COL1A2 antibody at 1/500 dilution Lane 1:Hela cell lysate Lane 2:Mouse liver tissue lysate Lane 3:Mouse lung tissue lysate Lane 4:Rat liver tissue lysate

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  Immunohistochemistry analysis of Collagen I Antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human COL1A2.
• Specificity: This antibody detects endogenous levels of Collagen I protein.
  (region surrounding Ser3)
• Formulation: Phosphate buffered saline (PBS),pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 125 kDa
• Gene Name: Homo sapiens collagen type I alpha 1 chain (COL1A1)
• Database Link:
  Entrez Gene 12842 MouseEntrez Gene 29393 RatEntrez Gene 1278 Human
  UniProt ID P08123
• Background: The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.
• Synonyms: COL1A2, Alpha-2 type I collagen

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion