S6K1 (RPS6KB1) Rabbit Polyclonal Antibody

CAT#: AP06552PU-N

S6K1 (RPS6KB1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p70 S6K antibody in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of p70 S6K antibody in paraffin-embedded human breast tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 190-240 of Human p70 S6K.
• Specificity: This antibody detects endogenous levels of p70 S6K protein.
  (region surrounding Asp223)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 50% glycerol
  Preservative: 0.09% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~59,70,85 kDa
• Gene Name: Homo sapiens ribosomal protein S6 kinase B1 (RPS6KB1), transcript variant 1
• Database Link:
  Entrez Gene 72508 MouseEntrez Gene 83840 RatEntrez Gene 6198 Human
• Background: p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal. Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinositide-3 kinase (PI-3K) and the target of rapamycin, FRAP/mTOR, a pathway distinct from the Ras/MAP kinase cascade. The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains.
• Synonyms: Ribosomal protein S6 kinase I, S6K1, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, Serine/threonine-protein kinase 14A

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, mTOR signaling pathway, TGF-beta signaling pathway