Desmin (DES) Rabbit Polyclonal Antibody

CAT#: AP06608PU-N

Desmin (DES) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Desmin antibody in extracts from PC12 cells.

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  Immunohistochemistry (IHC) analyzes of Desmin antibody in paraffin-embedded Human skeletal muscle tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on paraffin sections: 1/50-1/200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 420-470 of Human Desmin.
• Specificity: This antibody detects endogenous levels of Desmin protein.
  (region surrounding Ile448)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 54 kDa
• Gene Name: Homo sapiens desmin (DES)
• Database Link:
  Entrez Gene 13346 MouseEntrez Gene 64362 RatEntrez Gene 1674 Human
• Background: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain α-helical coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family such as the cytokeratins, in neoplasms including melanoma and breast carcinoma. Vimentin and Desmin, a related class III intermediate filament, are both expressed during skeletal muscle development.
• Synonyms: DES

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)