WNT1 Rabbit Polyclonal Antibody

CAT#: AP06617PU-N

WNT1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Wnt-1 antibody at 1/500 dilution Lane 1: HEK293T whole cell lysate Lane 2: H9C2 whole cell lysate Lane 3: sp2/0 whole cell lysate

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  Immunohistochemistry (IHC) analyzes of Wnt-1 antibody in paraffin-embedded Human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 301-350 of Human Wnt-1.
• Specificity: This antibody antibody detects endogenous levels of WNT1 protein.
  (region surrounding Gly331)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 41 kDa
• Database Link:
  Entrez Gene 7471 Human
  UniProt ID P04628
• Background: Products of the highly conserved Wnt gene family play key roles in regulating cellular growth and differentiation. The prototype member of the Wnt gene family, Wnt-1, is a cysteine-rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. β-catenin, a cadherin-binding cellular adhesion protein which also binds the tumor supressor gene APC, has been identified as a downstream target of a signal transduction pathway mediated by Wnt-1. Wnt-1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neural tube and adult spermatids. When improperly expressed in mammary tissue, Wnt-1 contributes to hyperplasia and tumorigenic progression. Wnt family members have been shown to interact with Sonic hedgehog (Shh) in vivo to induce myogenesis in somitic tissue.
• Synonyms: Proto-oncogene protein Wnt-1, INT1, INT-1