Osteoprotegerin (TNFRSF11B) Rabbit Polyclonal Antibody

CAT#: AP06631PU-N

Osteoprotegerin (TNFRSF11B) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of OPG antibody at 1/500 dilution Lane 1: HeLa whole cell lysate Lane 2: Mouse brain tissue lysate Lane 3: PC12 whole cell lysate Lane 4: Rat brain tissue lysate

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  Immunohistochemistry analysis of OPG antibody in paraffin-embedded human liver carcinoma tissue

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 1-50 of Human OPG.
• Specificity: This antibody detects endogenous levels of Osteoprotegerin protein.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~46 kDa
• Gene Name: tumor necrosis factor receptor superfamily member 11b
• Database Link:
  Entrez Gene 4982 Human
• Background: Bone morphogenesis and remodeling involve the formation of bone from osteoblasts and the resorption of bone by osteoclasts. The cytokine osteoprotegerin (OPG), also designated Osteoclastogenesis Inhibitory Factor (OCIF), is known to inhibit osteoclast formation. A secreted glycoprotein, OPG is a member of the TNF receptor family that increases bone density and volume. OPG is thought to inhibit osteoclastogenesis by disrupting the cell-to-cell signaling between osteoblastic stromal cells and osteoclast progenitors. OPG is known to bind to TRAIL, a death domain-containing protein, and to inhibit TRAIL apoptosis in Jurkat cells. OPG also binds to osteoclast differentiation factor (ODF), also known as TRANCE/RANKL, a membrane-bound protein belonging to the TNF ligand family. Both TNFα and TNFβ upregulate OPG expression, while the bone resorbing agent prostaglandin E2 downregulates OPG.
• Synonyms: OPG, OCIF