Creatine kinase M type (CKM) Rabbit Polyclonal Antibody

CAT#: AP06658PU-N

Creatine kinase M type (CKM) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Creatine Kinase M antibody at 1/500 dilution Lane 1:Hela whole cell lysate Lane 2:NIH-3T3 whole cell lysate Lane 3:H9C2 whole cell lysate

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  Immunohistochemistry (IHC) analyzes of Creatine Kinase M antibody in paraffin-embedded human heart tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Creatine Kinase M.
• Specificity: This antibody detects endogenous levels of Creatine Kinase M protein.
  (region surrounding Tyr14)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 43 kDa
• Gene Name: Homo sapiens creatine kinase, M-type (CKM)
• Database Link:
  Entrez Gene 12715 MouseEntrez Gene 24265 RatEntrez Gene 1158 Human
  UniProt ID P06732
• Background: Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKM isoform, predominant in skeletal muscle and heart tissue, is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
• Synonyms: Creatine kinase M-type, Creatine kinase M chain, M-CK, CKM, Creatine kinase MM

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways