Nuclear Factor 1 (NFIA) Rabbit Polyclonal Antibody

CAT#: AP06720PU-N

Nuclear Factor 1 (NFIA) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of NF-1 antibody in extracts from raw264.7 cells.

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  Immunohistochemistry (IHC) analysis of NF-1 antibody on paraffin-embedded sections.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to the amino acids 20-60 of Human NF-1A.
• Specificity: This antibody detects endogenous levels of NF-1 protein.
  (region surrounding Gln34)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 55 kDa
• Gene Name: Homo sapiens nuclear factor I/A (NFIA), transcript variant 1
• Database Link:
  Entrez Gene 18027 MouseEntrez Gene 25492 RatEntrez Gene 4774 Human
• Background: NF-1, also designated CTF, consists of a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, α-globin, HSP 70, GRP 78, Histone H1,myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.
• Synonyms: NFI-A, NF-I/A, CCAAT-box-binding transcription factor, TGGCA-binding protein, CTG

Reference Data

• Protein Families: Transcription Factors