CYP27A1 Rabbit Polyclonal Antibody

CAT#: AP06773PU-N

CYP27A1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CYP27A1 antibody at 1/500 dilution Lane 1:Hela whole cell lysate Lane 2:Raw264.7 whole cell lysate Lane 3:PC12 whole cell lysate

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  Immunohistochemistry (IHC) analyzes of CYP27A1 antibody in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 100-150 of Human CYP27A1.
• Specificity: This antibody detects endogenous levels of CYP27A1 protein.
  (region surrounding Glu131)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~60 kDa
• Gene Name: Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1)
• Database Link:
  Entrez Gene 1593 Human
  UniProt ID Q02318
• Background: The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity.Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans. CyPA is involved in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.
• Synonyms: CYP27, Cytochrome P450 27, Cytochrome P-450C27/25, Sterol 26-hydroxylase, Sterol 27-hydroxylase, Vitamin D3-25-hydroxylase

Reference Data

• Protein Families: Druggable Genome, P450
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis