Collagen III (COL3A1) Rabbit Polyclonal Antibody

CAT#: AP07843PU-N

Collagen III (COL3A1) rabbit polyclonal antibody, Aff - Purified

Product Images

Staining COL3A1 in Kidney by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.

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  Staining COL3A1 in Testis by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 1/5000 - 1/10000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Immunoprecipitation.
  Western Blot.
• Reactivities: Bovine, Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Collagen Type III from Human and Bovine placenta
• Specificity: This antibody detects Collagen Type III from Placenta.
• Formulation: 0.02M Potasium Phopshate, pH 7.2, 0.15M NaCl
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Preservative: 0.01% (w/v) Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 1281 Human
• Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
• Synonyms: COL3A1