Collagen VI (COL6A1) Rabbit Polyclonal Antibody

CAT#: AP07844PU-N

Collagen VI (COL6A1) rabbit polyclonal antibody, Aff - Purified

Product Images

Collagen type VI antibody staining of Formalin-Fixed Paraffin-Embedded Human Breast tissue.

• 

  Collagen type VI antibody staining of Formalin-Fixed Paraffin-Embedded Human Lung tissue.

Specifications

Product Data

• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunofluorescence.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Immunoprecipitation.
  Western Blot.
• Reactivities: Bovine, Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Collagen Type VI from Human and Bovine placenta
• Specificity: This antibody detects Collagen, Type VI from Human and Bovine Placenta.
  Other species not tested.
• Formulation: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; pH 8.0 with 0.01% (w/v) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1291 Human
• Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
• Synonyms: COL6A1