Tubulin (TUBA1B) (427-441) (Loading Control) Rabbit Polyclonal Antibody

CAT#: AP07868PU-N

Tubulin (TUBA1B) (427-441) (Loading Control) rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Staining Tubulin alpha in Brain, cortex by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/5000 - 1/25000.
  Immunofluorescence.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 1/500 - 1/3000.
• Reactivities: Bovine, Chicken, Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to Amino acids 427-441 of Human alpha Tubulin.
• Specificity: This antibody makes an excellent Loading Control.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: 0.85 mg/ml
• Purification: Immunoaffinity Chromatography.
• Gene Name: Homo sapiens tubulin alpha 1b (TUBA1B)
• Database Link:
  Entrez Gene 22143 MouseEntrez Gene 500929 RatEntrez Gene 10376 Human
• Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.
• Synonyms: Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection