Myosin Light Chain 2 (MYL2) (pSer19) Rabbit Polyclonal Antibody

CAT#: AP08007PU-N

Myosin Light Chain 2 (MYL2) (pSer19) rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extracts from 293 cells untreated or treated with starvation using Myosin Light Chain 2 pSer18 Antibody.

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myosin Light Chain 2 (pSer18 Antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 19 (A-T-Sp-N-V) derived from Human Myosin Light Chain 2.
• Specificity: This antibody detects endogenous levels of Myosin Light Chain 2 only when phosphorylated at Serine 19.
• Formulation: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 50% Glycerol
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 18 kDa
• Gene Name: myosin light chain 2
• Database Link:
  Entrez Gene 4633 Human
• Background: Myosin light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
• Synonyms: MLC-2v, MLC-2