Synaptotagmin 1 (SYT1) Rabbit Polyclonal Antibody

CAT#: AP08066PU-S

Synaptotagmin 1 (SYT1) rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extract from Hela cells using Synaptotagmin I/II Antibody (Lane 1 and 2).

• 

  Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using Synaptotagmin I/II antibody (Red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500~1/1000.
  Immunofluorescence: 1/100~1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Synaptotagmin I/II around the phosphorylation site of Threonine 202/199 (R-K-Tp-L-N).
• Specificity: This antibody detects endogenous levels of total Synaptotagmin I/II protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific immunogen.
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens synaptotagmin 1 (SYT1), transcript variant 2
• Database Link:
  Entrez Gene 20979 MouseEntrez Gene 25716 RatEntrez Gene 6857 Human
• Background: Synaptotagmin 1 is a 60kDa member of the synaptotagmin family, expressed in membranes of synaptic vesicles in rostral, phylogenetically younger brain regions and in some endocrine tissues. Ca2+ is bound by synaptogamin whose C2 domains then bind phospholipids, trafficking vesicles to the synaptic membrane and exocytosis. This results in fast synchronous neurotransmitter release. Synaptotagmin also binds neurexins, syntaxin and AP2 independently of Ca2+ binding. Phosphorylation of synaptotagmin by protein kinases is thought to modulate its activity.
  Synaptotagmin 2 belongs to the synaptotagmin family of multi-domained, integral membrane proteins of synaptic vesicles which are thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
• Synonyms: Synaptotagmin I/II, SYT1/SYT2

Reference Data

• Protein Families: Secreted Protein, Transmembrane