HA Epitope Tag (YPYDVPDYA) Rabbit Polyclonal Antibody

CAT#: AP08109PU-N

HA Epitope Tag (YPYDVPDYA) rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extracts from 293 cells transfected with HA-tagged Akt using HA-Tag Polyclonal Antibody AP08109PU.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot (1/500-1/1000).
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced by immunizing rabbit with synthesized peptide containing the Influenza hemagglutinin epitope (Y-P-Y-D-V-P-D-Y-A).
• Specificity: This polyclonal antibody detects HA-tagged proteins overexpressed in cells.
  It recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected cells.
• Formulation: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction.
  Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Immunoaffinity Chromatography using epitope-specific peptide.
• Background: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.