Carbonic Anhydrase II (CA2) Rabbit Polyclonal Antibody

CAT#: AP08499HR-N

Carbonic Anhydrase II (CA2) rabbit polyclonal antibody, HRP

Product Images

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/1000 - 1/6000.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Carbonic Anhydrase II (Human Erythrocytes).
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti Rabbit serum, as well as purified and partially purified Carbonic Anhydrase II (Human Erythrocytes).
• Formulation: PBS, pH 7.2 containing 0.01% Gentamicin Sulfate as preservative and 10 mg/ml BSA as stabilizer, before the addition of Glycerol to 40%
  Label: HRP
  State: Liquid purified Ig fraction.
  Label: Horseradish Peroxidase
• Concentration: lot specific
• Purification: Ion Exchange Chromatography.
• Conjugation: HRP
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 760 Human
• Background: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20µM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.
• Synonyms: Carbonic anhydrase II, Carbonate dehydratase II, Carbonic anhydrase C, CA2, CA-II, CAC