CMG1 (IFT74) (588-600) Goat Polyclonal Antibody

CAT#: AP08569PU-N

CMG1 (IFT74) (588-600) goat polyclonal antibody, Aff - Purified

Product Images

Antibody staining (0.3 ug/ml) of 293 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using Chemiluminescence.

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  Liver: Formalin-Fixed Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/16000.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot.
• Reactivities: Human, Monkey
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-terminus of Human IFT74
• Specificity: Recognizes IFT74/CMG1 at aa 588-600.
• Formulation: Tris saline buffer, pH 7.3 containing 0.5% BSA as stabilizer and 0.02% Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 0.5 mg/ml
• Purification: Immunoaffinity Chromatography
• Database Link:
  Entrez Gene 80173 Human
• Background: Capillary morphogenesis protein 1 (CMG1) is a 600 amino acid protein and has a calculated molecular mass of 65 kD. CMG1 contains a coiled-coil domain (which shares 24% identity with myosin heavy chain sequences from various species) and several putative phosphorylation sites. Bell et al. (2001) determined that CMG1 was 1 of several transcripts upregulated by umbilical vein endothelial cells during capillary morphogenesis in 3-dimensional collagen matrices. RT-PCR detected highest expression in adult and fetal kidney and lower expression in adult heart, placenta, lung, liver, and pancreas, and in fetal heart, lung, and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus, and spleen. Transfection into epithelial tumor cells revealed targeting of fluorescent-tagged CMG1 to an intracellular vesicular compartment.
• Synonyms: CCDC2, CMG1, CMG-1