ALDH1A1 (476-488) Goat Polyclonal Antibody

CAT#: AP08840PU-N

ALDH1A1 (476-488) goat polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of Human Liver Lysate (35 ug protein in RIPA buffer) using ALDH1A1 antibody. Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Formalin-Fixed Paraffin-Embedded Human Testis stained with ALDH1A1 antibody at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/32000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 1/32000, 1 - 3 µg/ml.
• Reactivities: Bovine, Human, Sheep
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from an internal region of Human ALDH1A1
• Specificity: This antibody recognizes Aldehyde Dehydrogenase 1 (ALDH1A1) at 476-488.
• Formulation: Tris saline buffer, pH 7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 216 Human
• Background: ALDH1A1 is an aldehyde dehydrogenase able to oxidize a wide variety of aliphatic aldehydes (retinaldehyde, acetaldehyde, etc.) to the corresponding carboxylic acids (retinoic acid, acetic acid, etc.). ALDH1A1 (also known as RALDH1, ALDH1, or AHD2) is highly expressed in the dorsal retina, ventral midbrain (dopaminergic neurons), and hematopoietic stem cells. ALDH1A1 is involved in retinoic acid (RA) synthesis during vertebrate embryogenesis. ALDH1A1 is first detected at E9.0-E10.5 in cranial tissues (ventral mesencephalon, dorsal retina, thymic primordia, optic vesicles) and in the mesonephros. ALDH1A1 is also of interest in Parkinson's Disease (PD) being expressed in the the A9 dopaminergic (DA) neuronal group projecting to the dorsal striatum; this is the most vulnerable site in PD (Chung et al, 2005). Some studies also identify ALDH1A1 as a marker of cancer stem cells. Studies of breast cancer tissues showed that those that expressed a form of ALDH1A1 had the worst outcomes.
• Synonyms: Retinal dehydrogenase 1, RALDH1, ALDH1A1, ALDC, PUMB1, Aldehyde dehydrogenase cytosolic, ALHDII, ALDH-E1