Iba1 (AIF1) (149-161) Goat Polyclonal Antibody

CAT#: AP08912PU-N

Iba1 (149-161) goat polyclonal antibody, Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Product Images

AIF1 Antibody (0.5 ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Formalin-Fixed, Paraffin-Embedded Human Lung staining using AIF1 Antibody at 5 ug/ml after heat-induced antigen retrieval.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: IHC
  IHC-P (5 µg/ml)
  Peptide-ELISA (1:128000)
  WB (0.3 - 1 µg/ml)
• Reactivities: Hamster, Human, Monkey, Mouse, Porcine, Rat, Bovine, Equine, Goat, Rabbit, Pig
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-TGPPAKKAISELP, from the C-Terminus of protein sequence according to NP_116573.1NP_001614.3.
• Specificity: Human AIF1 / IBA1. This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
• Formulation: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
• Stability: 12 months from date of despatch
• Gene Name: allograft inflammatory factor 1
• Database Link:
  Entrez Gene 199 Human
  UniProt ID P55008
• Background: IBA1 antibody AP08912PU-N is an unconjugated goat polyclonal antibody to IBA1 (AIF1) (aa149-161) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
• Synonyms: AIF1, AIF-1, Em:AF129756.17, G1, IRT-1, Protein G1, IBA1, IRT1
• Note: Immunohistochemistry: LS-B2645 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2645 was determined to be 5 ug/ml.