SARS M (C-term) Rabbit Polyclonal Antibody

CAT#: AP09091SU-N

SARS M (C-term) rabbit polyclonal antibody, Serum

Product Images

Immunofluorescence Microscopy using anti-SARS-CoV Membrane protein antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX (611-104-122) was used. Personal Communication, Eric Snijder, Leiden University Medical Cen

Specifications

Product Data

• Applications: EM, IF, IP, WB
• Recommended Dilution: Immunoprecipitaion: 1:60.
  Western blot: 1:1,000 (a band of approximately 25 kDa in size corresponding to SARS-CoV membrane protein in the appropriate cell lysate or extract).
  Immunoelectron microscopy: 1:200.
  Immunoflourescence: 1:1,000 (Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min).
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: BSAcoupled Synthetic peptide corresponding to the C-terminus (amino acid residues 204-221) of the SARS Coronavirus Membrane protein
• Specificity: This antibody is directed against SARS-Coronavirus membrane protein. Cross reactivity with homologues from other sources has not been determined.
• Formulation: 0.01% (w/v) Sodium Azide
  State: Serum
  State: Liquid sterile filtered neat antiserum
• Concentration: 85 mg/ml (by Refractometry)
• Background: The coronavirus membrane protein is a major structural component of the viral envelope and a determinant of virus assembly.
• Synonyms: SARS-CoV M, Severe acute respiratory syndrome coronavirus Membrane protein, M protein, Membrane glycoprotein, Matrix protein, Matrix glycoprotein, E1 glycoprotein