Luciferase (Photinus pyralis) Goat Polyclonal Antibody

CAT#: AP09104BT-S

Luciferase (Photinus pyralis) goat polyclonal antibody, Biotin, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot : 1/1,000 - 1/5,000.
  ELISA : 1/10,000 - 1/25,000.
  Firefly-luciferase produces a green light with a wavelength of 562 nm; Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract. Anti-Luciferase has been assayed against 1.0 µg of Luciferase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/80,000 to 1/310,000 of the reconstitution concentration is suggested.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Luciferase from Photinus pyralis (Firefly)
• Specificity: This antibody reacts to Luciferase.
  Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: Biotin
  State: Purified
  State: Lyophilized IgG fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
  For extended storage, mix product with glycerol to 50% final.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.