Luciferase (Photinus pyralis) Goat Polyclonal Antibody

CAT#: AP09104BT-S

Luciferase (Photinus pyralis) goat polyclonal antibody, Biotin, Purified


USD 525.00

3 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Western blot : 1/1,000 - 1/5,000.
ELISA : 1/10,000 - 1/25,000.
Firefly-luciferase produces a green light with a wavelength of 562 nm; Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract. Anti-Luciferase has been assayed against 1.0 µg of Luciferase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature.  A working dilution of 1/80,000 to 1/310,000 of the reconstitution concentration is suggested.
Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Luciferase from Photinus pyralis (Firefly)
Specificity This antibody reacts to Luciferase.
Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)].  No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: Biotin
State: Purified
State: Lyophilized IgG fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
For extended storage, mix product with glycerol to 50% final.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation Biotin
Background Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Reference Data

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