Luciferase (Photinus pyralis) Goat Polyclonal Antibody

CAT#: AP09104FC-S

Luciferase (Photinus pyralis) goat polyclonal antibody, FITC

Specifications

Product Data

• Applications: FC, IF
• Recommended Dilution: Immunofluorescence: 1/500 - 1/2500.
  
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Luciferase (Photinus pyralis (Firefly))
• Specificity: This antibody reacts to Luciferase.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
  Label: FITC
  State: Lyophilized IgG fraction
  Label: Fluorescein isothiocyanate
  Absorption emission: 495 nm / 528 nm
  Molar radio: 2.5 moles FITC per mole of Goat IgG
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
  For extended storage, mix product with glycerol to 50% final
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Ion exchange chromatography
• Conjugation: FITC
• Predicted Protein Size: 390 kDa.
• Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.