Luciferase (Photinus pyralis) Goat Polyclonal Antibody
CAT#: AP09104HR-S
Luciferase (Photinus pyralis) goat polyclonal antibody, HRP, Purified
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommended Dilution | Western blot : 1/5,000 - 1/10,000. ELISA : 1/50,000 - 1/100,000. Firefly-luciferase produces a green light with a wavelength of 562 nm; Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract. Anti-Luciferase has been assayed against 1.0 µg of Luciferase in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/50,000 to 1/200,000 of the reconstitution concentration is suggested. |
Host | Goat |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Luciferase from Photinus pyralis (Firefly) |
Specificity | This antibody reacts to Luciferase. Immunoelectrophoresis give a single precipitin arc against anti-perosidase, anti-goat serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized IgG fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | HRP |
Background | Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
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