Protein A Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, FC, IF, IHC |
Recommended Dilution | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Flow cytometry: 1:2,000 - 1:10,000. Immunoflourescence: 1:500 - 1:2,500. |
Host | Goat |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Protein A [Staphylococcus aureus] |
Specificity | This antibody detects Protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus], anti-Goat IgG and anti-Goat Serum. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 as buffer, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer, 0.01% (w/v) Sodium Azide as preservative Label: Texas Red State: Lyophilized Ig fraction Label: (TM) Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption emission: 596 nm / 620 nm Molar radio: 1.5 moles Texas Red(TM) per mole of Goat IgG |
Reconstitution Method | Restore with 2.0 ml of deionized water (or equivalent). |
Concentration | 10.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | Texas Red |
Reference Data |
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