Protein G Rabbit Polyclonal Antibody

CAT#: AP09166FC-N

Protein G rabbit polyclonal antibody, FITC

Specifications

Product Data

• Applications: FC, IF
• Recommended Dilution: Immunofluorescence: 1/500 - 1/2500.
  Flow Cytometry: 1/2000 - 1/10000.
• Reactivities: Streptococcus
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Protein G [Streptococcus species]
• Specificity: This antibody reacts to Protein G.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% Thimerosal as preservative
  Label: FITC
  State: Lyophilized IgG fraction
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Ion exchange chromatography
• Conjugation: FITC
• Background: Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
  Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.