Protein G Rabbit Polyclonal Antibody

CAT#: AP09166HR-N

Protein G rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western Blot.
  Dot Blot.
  ELISA: 1/20000 to 1/100000.
  Immunohistochemistry.
• Reactivities: Streptococcus
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Protein G [Streptococcus species]
• Specificity: This antibody reacts to Protein G.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative
  Label: HRP
  State: Lyophilized IgG fraction
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
  For extended storage reconstitute product with 50% glycerol.
• Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Ion exchange chromatography
• Conjugation: HRP
• Background: Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
  Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.