Biotin Rabbit Polyclonal Antibody

CAT#: AP09175FC-N

Biotin rabbit polyclonal antibody, FITC


USD 3,925.00

3 Days*

Size
    • 20 mg

Product Images

Specifications

Product Data
Applications FC, IF, IHC
Recommended Dilution
Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. 
Recommended Dilutions:
Immunofluorescence: 1/500-1/2,500.
Flow Cytometry:1/2,000-1/10,000.
Note: All assays should be optimized by the user.
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Biotin conjugated Keyhole Limpet Hemocyanin (KLH)
Specificity
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. 
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Albumin. 
Formulation 0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4
Label: FITC
State: Lyophilized IgG fraction
Stabilizer: 10 mg/ml BSA (IgG and Protease free)
Preservative: 0.01% (w/v) Thimerisol
Absorption emission: 495 nm / 528 nm
Molar radio: 2.7 moles Fluorescein (FITC) per mole of IgG
Reconstitution Method Restore with 2.0 ml of deionized water (or equivalent).
Concentration 10.0 mg/ml (by UV absorbance at 280 nm)
Conjugation FITC
Background Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Synonyms Vitamin B7, Vitamin H
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.