Biotin Rabbit Polyclonal Antibody

CAT#: AP09180TR-N

Biotin rabbit polyclonal antibody, Texas Red

Specifications

Product Data

• Applications: ELISA, FC
• Recommended Dilution: Immunofluorescence.
  Flow Cytometry.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Specificity: This antibody reacts to Biotin.
• Formulation: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
  Label: Texas Red
  State: Lyophilized IgG fraction
  Label: (TM) Sulfonyl Chloride (Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 2.1 moles Texas Red(TM) per mole of Rabbit IgG
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
  For extended storage reconstitute product with 50% glycerol instead of water
• Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)
• Conjugation: Texas Red
• Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
• Synonyms: Vitamin B7, Vitamin H
• Note: Texas Red(TM) is a registered trademark of Molecular Probes Inc.