FITC Goat Polyclonal Antibody

CAT#: AP09203BT-N

FITC goat polyclonal antibody, Biotin


USD 675.00

3 Days*

Size
    • 1 mg

Product Images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution ELISA: 1/115,000.
Western Blot: 1/1,000 - 1/3,000.
Immunohistochemistry.
Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Fluorescein conjugated to Goat IgG
Specificity This antibody reacts to Fluorescein.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
Label: Biotin
State: Lyophilized purified Ig
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio: 10-20 BAC molecules per Goat IgG molecule
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
For extended storage mix product with 50% glycerol.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Affinity chromatography
Conjugation Biotin
Background Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
Synonyms Fluorescein Isothiocyanate
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.