FITC Goat Polyclonal Antibody

CAT#: AP09203BT-N

FITC goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/115,000.
  Western Blot: 1/1,000 - 1/3,000.
  Immunohistochemistry.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fluorescein conjugated to Goat IgG
• Specificity: This antibody reacts to Fluorescein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
  Label: Biotin
  State: Lyophilized purified Ig
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Goat IgG molecule
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
  For extended storage mix product with 50% glycerol.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Affinity chromatography
• Conjugation: Biotin
• Background: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
• Synonyms: Fluorescein Isothiocyanate