FITC Goat Polyclonal Antibody

CAT#: AP09203TC-N

FITC goat polyclonal antibody, TRITC

Specifications

Product Data

• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Immunofluorescence.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fluorescein conjugated to Keyhole Limpet Hemocyanin (KLH)
• Specificity: This antibody reacts to Fluorescein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
  Label: TRITC
  State: Lyophilized purified Ig
  Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 2.0 moles TRITC per mole of Goat IgG
• Reconstitution Method: Restore with 1,0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Affinity chromatography
• Conjugation: TRITC
• Background: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
• Synonyms: Fluorescein Isothiocyanate