KLH Rabbit Polyclonal Antibody

CAT#: AP09215TC-N

KLH rabbit polyclonal antibody, Rhodamine

Specifications

Product Data

• Applications: FC, IF
• Recommended Dilution: Flow Cytometry: 1/2000 - 1/10000.
  Immunofluorescence: 1/500 - 1/2500.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: KLH (Keyhole Limpet Hemocyanin)
• Specificity: This antibody reacts to Keyhole Limpet Hemocyanin (KLH).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer
  Label: Rhodamine
  State: Lyophilized
  Label: Tetramethylrhodamine isothiocyanate (TRITC)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 2.8 moles TRITC per mole of Rabbit IgG
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
  For extended storage mix product with glycerol to 50%
• Concentration: 1,0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: Rhodamine
• Background: KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
• Synonyms: Keyhole Limpet Hemocyanine